This product is L-2-amino-3-methylvaleric acid. Calculated as dry product, the content of C6H13NO2 is less than 98.5%.
[Properties] This product is white crystal or crystalline powder; odorless.
This product is slightly soluble in water and almost insoluble in ethanol or ether.
Take this product, accurately weigh it, add 6mol/L hydrochloric acid solution to dissolve and dilute quantitatively to make a solution containing about 40mg per 1ml, measured according to law (General Rule 0621), the specific rotation is +38.9° to +41.8° .
[Identification] (1) Take the appropriate amount of this product and the isoleucine reference substance, respectively add water to dissolve and dilute to make a solution containing about 0.4mg per 1ml, as the test solution and reference solution. According to the chromatographic condition test under other amino acids, the position and color of the main spots in the test solution should be the same as the main spots in the reference solution.
(2) The infrared absorption spectrum of this product should be consistent with the control spectrum (spectrum set 894).
[Check] Acidity Take 0.20g of this product, add 20ml of water to dissolve it, and measure it according to law (General Rule 0631). The pH should be 5.5-6.5.
Light transmittance of the solution Take 0.5g of this product, add 20ml of water to dissolve it, and measure the light transmittance at a wavelength of 430nm according to ultraviolet-visible spectrophotometry (General Rule 0401), and it shall not be less than 98.0%.
Chloride Take 0.25g of this product and check according to law (General Rule 0801). Compared with the control solution made of 5.0ml standard sodium chloride solution, it should not be more concentrated (0.02%).
Sulfate Take 1.0g of this product and check according to the law (General Rule 0802). Compared with the control solution made of 2.0ml of standard potassium sulfate solution, it should not be more concentrated (0.02%).
Ammonium salt Take 0.10g of this product and check according to law (General Rule 0808). Compared with the control solution made of 2.0ml standard ammonium chloride solution, it should not be deeper (0.02%).
For other amino acids, take appropriate amount of this product, dissolve it in water and dilute it to make a solution containing about 20mg per 1ml, as the test solution; accurately measure 1ml, put it in a 200ml measuring flask, dilute to the mark with water, shake it up, and use it as a control solution ; Take another appropriate amount of isoleucine reference substance and valine reference substance, put them in the same measuring bottle, add water to dissolve and dilute to make a solution containing about 0.4mg per 1ml, as a system suitability solution. According to the thin-layer chromatography (general rule 0502) test, absorb 5μ1 of each of the above three solutions, and spot them on the same silica gel G thin-layer plate, using n-butanol-water-glacial acetic acid (3:1:1) as the developing agent, Unfold, dry, spray with ninhydrin acetone solution (1→50), heat at 80°C until spots appear, check immediately. The control solution should show one clear spot, and the system suitability solution should show two completely separated spots. If the test solution shows impurity spots, its color should not be darker (0.5%) compared with the main spot of the control solution.
Loss on drying Take this product and dry it at 105°C for 3 hours. The weight loss should not exceed 0.2% (General Rule 0831).
Residue on ignition Take 1.0g of this product and inspect it according to law (General Rule 0841). The remaining residue shall not exceed 0.1%.
Iron salt Take 2.0g of this product and inspect it according to law (General Rule 0807). Compared with the control solution made of 2.0ml of standard iron solution, it should not be deeper (0.001%).
Heavy metals Take the residues left over under the item of residues on ignition, and inspect them according to law (General Rule 0821 Law 2), and the content of heavy metals shall not exceed 10 parts per million.
Arsenic salt take 2.0g of this product, add 5ml of water, add 1ml of sulfuric acid and 10ml of sulfurous acid, heat on a water bath until the volume is about 2ml, add 5ml of water, add dropwise ammonia test solution until the indicator solution of phenolphthalein is neutral, add 5ml of hydrochloric acid, Add water to make 28ml, and check according to law (General Rule 0822 First Law), and it should meet the requirements (0.0001%).
Finely average endotoxin. Take this product and check according to law (General Rule 1143). The amount of endotoxin contained in 1g of isoleucine should be less than 20EU. (For injection)
[Content determination] Take about 0.10g of this product, accurately weigh it, add 1ml of anhydrous formic acid to dissolve, add 25ml of glacial acetic acid, and titrate with perchloric acid titrant (0.1mol/L) according to the potentiometric titration method (General Rule 0701) , And correct the results of the titration with a blank test. Each 1ml of perchloric acid titrant (0.1mol/L) is equivalent to 13.12mg of C6H13NO2.
[Category] Amino acid drugs.
[Storage] shading, sealed and preserved.
About us
Biologists and Chemists at BOC Sciences offer extensive tech support to our customers for using the products. You may expect personalized and attentive care from our support staff answering your order and technical inquiries. We also provide custom services including amino aldehydes, peptide inhibitor , 3 boc amino propyl bromide and boc glycine methyl ester.
